Microscopy:Article Title: Labeling PIEZO2 activity in the peripheral nervous system
Article Snippet: .. Sakura Cat.# 4583 Gold anti-fade mounting medium Thermo Fisher Cat.# S36939 Fluoromount G mounting medium with DAPI Thermo Fisher Cat.# 00-4959-52 Superfrost Plus microscope slides Fisher Cat.# 12-550-15 Lipofectamine 2000 Thermo Fisher Cat.# 11668019 PEI MAX Polysciences Cat.# 24765 Dmem, high glucose, pyruvate Thermo Fisher Cat.# 11995073 Penicillin-Streptomycin (10,000 U/mL) Thermo Fisher Cat.# 15140122 Matrigel Corning Cat.# 354234 MEM (Minimum Essential Medium) Thermo Fisher Cat.# 11095080 Gibco Normal Goat Serum Thermo Fisher Cat.# PCN5000 Donkey Serum Sigma Cat.# D9663 Experimental models: Cell lines Neuro2A-P1ko Gary Lewin 56 N/A Piezo1 knock-out HEK293T (HEK P1KO) ATCC Cat.# CRL-3519 Experimental models: Organisms/strains C57BL/6J Jackson Laboratories RRID:IMSR_JAX:000664 CGRP cre+ ;GFP Mark J. Zylka 35 N/A HoxB8 Cre+ ;Piezo2 f/f Previously reported in 4 N/A Phox2b Cre ; Piezo2 f/f Previously reported in 6 N/A Recombinant DNA hPIEZO2 - codon-optimized-T2A-BFP-WPRE (“PIEZO2”) This paper, Table S1 N/A hPIEZO2 -codon-optimized_mCherry-WPRE This paper, Table S2 N/A hPIEZO2_R2686H -codon-optimized-T2A-BFP-WPRE ( “PIEZO2 R2686H ) This paper, Table S3 N/A hPiezo1-pIRES2-EGFP Previously reported in 57 N/A hPiezo1-pIRES2-tdTomato This paper, Table S4 N/A Rat TRPV1 Previously reported in 58 N/A Software and algorithms FIJI (ImageJ) Schindelin et. al. (2012) doi: 10.1038/nmeth.2019 RRID:SCR_002285 Imaris (version 9.9.1) Oxford Instruments RRID:SCR_007370 Prism (GraphPad 9.2.0) GraphPad Software RRID:SCR_002798 Microsoft Excel Microsoft RRID:SCR_016137 FlowJo 10.7.2 BD RRID:SCR_008520 Igor Pro 8.02 Wavemetrics RRID:SCR_000325 pClamp software (version 10.7) Molecular Devices RRID:SCR_011323 Patchmaster HEKA Elektronik RRID:SCR_000034 Other EPC10 amplifier HEKA Elektronik N/A HSPC-1 ALA Scientific Instruments N/A MultiClamp700A amplifier Molecular Devices N/A DigiData1550 Molecular Devices N/A Open in a separate window Key resources table FM 1–43 is a fluorescent dye that labels mouse sensory neurons in vivo. ..
Knock-Out:Article Title: Labeling PIEZO2 activity in the peripheral nervous system
Article Snippet: .. Sakura Cat.# 4583 Gold anti-fade mounting medium Thermo Fisher Cat.# S36939 Fluoromount G mounting medium with DAPI Thermo Fisher Cat.# 00-4959-52 Superfrost Plus microscope slides Fisher Cat.# 12-550-15 Lipofectamine 2000 Thermo Fisher Cat.# 11668019 PEI MAX Polysciences Cat.# 24765 Dmem, high glucose, pyruvate Thermo Fisher Cat.# 11995073 Penicillin-Streptomycin (10,000 U/mL) Thermo Fisher Cat.# 15140122 Matrigel Corning Cat.# 354234 MEM (Minimum Essential Medium) Thermo Fisher Cat.# 11095080 Gibco Normal Goat Serum Thermo Fisher Cat.# PCN5000 Donkey Serum Sigma Cat.# D9663 Experimental models: Cell lines Neuro2A-P1ko Gary Lewin 56 N/A Piezo1 knock-out HEK293T (HEK P1KO) ATCC Cat.# CRL-3519 Experimental models: Organisms/strains C57BL/6J Jackson Laboratories RRID:IMSR_JAX:000664 CGRP cre+ ;GFP Mark J. Zylka 35 N/A HoxB8 Cre+ ;Piezo2 f/f Previously reported in 4 N/A Phox2b Cre ; Piezo2 f/f Previously reported in 6 N/A Recombinant DNA hPIEZO2 - codon-optimized-T2A-BFP-WPRE (“PIEZO2”) This paper, Table S1 N/A hPIEZO2 -codon-optimized_mCherry-WPRE This paper, Table S2 N/A hPIEZO2_R2686H -codon-optimized-T2A-BFP-WPRE ( “PIEZO2 R2686H ) This paper, Table S3 N/A hPiezo1-pIRES2-EGFP Previously reported in 57 N/A hPiezo1-pIRES2-tdTomato This paper, Table S4 N/A Rat TRPV1 Previously reported in 58 N/A Software and algorithms FIJI (ImageJ) Schindelin et. al. (2012) doi: 10.1038/nmeth.2019 RRID:SCR_002285 Imaris (version 9.9.1) Oxford Instruments RRID:SCR_007370 Prism (GraphPad 9.2.0) GraphPad Software RRID:SCR_002798 Microsoft Excel Microsoft RRID:SCR_016137 FlowJo 10.7.2 BD RRID:SCR_008520 Igor Pro 8.02 Wavemetrics RRID:SCR_000325 pClamp software (version 10.7) Molecular Devices RRID:SCR_011323 Patchmaster HEKA Elektronik RRID:SCR_000034 Other EPC10 amplifier HEKA Elektronik N/A HSPC-1 ALA Scientific Instruments N/A MultiClamp700A amplifier Molecular Devices N/A DigiData1550 Molecular Devices N/A Open in a separate window Key resources table FM 1–43 is a fluorescent dye that labels mouse sensory neurons in vivo. ..
Recombinant:Article Title: Labeling PIEZO2 activity in the peripheral nervous system
Article Snippet: .. Sakura Cat.# 4583 Gold anti-fade mounting medium Thermo Fisher Cat.# S36939 Fluoromount G mounting medium with DAPI Thermo Fisher Cat.# 00-4959-52 Superfrost Plus microscope slides Fisher Cat.# 12-550-15 Lipofectamine 2000 Thermo Fisher Cat.# 11668019 PEI MAX Polysciences Cat.# 24765 Dmem, high glucose, pyruvate Thermo Fisher Cat.# 11995073 Penicillin-Streptomycin (10,000 U/mL) Thermo Fisher Cat.# 15140122 Matrigel Corning Cat.# 354234 MEM (Minimum Essential Medium) Thermo Fisher Cat.# 11095080 Gibco Normal Goat Serum Thermo Fisher Cat.# PCN5000 Donkey Serum Sigma Cat.# D9663 Experimental models: Cell lines Neuro2A-P1ko Gary Lewin 56 N/A Piezo1 knock-out HEK293T (HEK P1KO) ATCC Cat.# CRL-3519 Experimental models: Organisms/strains C57BL/6J Jackson Laboratories RRID:IMSR_JAX:000664 CGRP cre+ ;GFP Mark J. Zylka 35 N/A HoxB8 Cre+ ;Piezo2 f/f Previously reported in 4 N/A Phox2b Cre ; Piezo2 f/f Previously reported in 6 N/A Recombinant DNA hPIEZO2 - codon-optimized-T2A-BFP-WPRE (“PIEZO2”) This paper, Table S1 N/A hPIEZO2 -codon-optimized_mCherry-WPRE This paper, Table S2 N/A hPIEZO2_R2686H -codon-optimized-T2A-BFP-WPRE ( “PIEZO2 R2686H ) This paper, Table S3 N/A hPiezo1-pIRES2-EGFP Previously reported in 57 N/A hPiezo1-pIRES2-tdTomato This paper, Table S4 N/A Rat TRPV1 Previously reported in 58 N/A Software and algorithms FIJI (ImageJ) Schindelin et. al. (2012) doi: 10.1038/nmeth.2019 RRID:SCR_002285 Imaris (version 9.9.1) Oxford Instruments RRID:SCR_007370 Prism (GraphPad 9.2.0) GraphPad Software RRID:SCR_002798 Microsoft Excel Microsoft RRID:SCR_016137 FlowJo 10.7.2 BD RRID:SCR_008520 Igor Pro 8.02 Wavemetrics RRID:SCR_000325 pClamp software (version 10.7) Molecular Devices RRID:SCR_011323 Patchmaster HEKA Elektronik RRID:SCR_000034 Other EPC10 amplifier HEKA Elektronik N/A HSPC-1 ALA Scientific Instruments N/A MultiClamp700A amplifier Molecular Devices N/A DigiData1550 Molecular Devices N/A Open in a separate window Key resources table FM 1–43 is a fluorescent dye that labels mouse sensory neurons in vivo. ..
Software:Article Title: Labeling PIEZO2 activity in the peripheral nervous system
Article Snippet: .. Sakura Cat.# 4583 Gold anti-fade mounting medium Thermo Fisher Cat.# S36939 Fluoromount G mounting medium with DAPI Thermo Fisher Cat.# 00-4959-52 Superfrost Plus microscope slides Fisher Cat.# 12-550-15 Lipofectamine 2000 Thermo Fisher Cat.# 11668019 PEI MAX Polysciences Cat.# 24765 Dmem, high glucose, pyruvate Thermo Fisher Cat.# 11995073 Penicillin-Streptomycin (10,000 U/mL) Thermo Fisher Cat.# 15140122 Matrigel Corning Cat.# 354234 MEM (Minimum Essential Medium) Thermo Fisher Cat.# 11095080 Gibco Normal Goat Serum Thermo Fisher Cat.# PCN5000 Donkey Serum Sigma Cat.# D9663 Experimental models: Cell lines Neuro2A-P1ko Gary Lewin 56 N/A Piezo1 knock-out HEK293T (HEK P1KO) ATCC Cat.# CRL-3519 Experimental models: Organisms/strains C57BL/6J Jackson Laboratories RRID:IMSR_JAX:000664 CGRP cre+ ;GFP Mark J. Zylka 35 N/A HoxB8 Cre+ ;Piezo2 f/f Previously reported in 4 N/A Phox2b Cre ; Piezo2 f/f Previously reported in 6 N/A Recombinant DNA hPIEZO2 - codon-optimized-T2A-BFP-WPRE (“PIEZO2”) This paper, Table S1 N/A hPIEZO2 -codon-optimized_mCherry-WPRE This paper, Table S2 N/A hPIEZO2_R2686H -codon-optimized-T2A-BFP-WPRE ( “PIEZO2 R2686H ) This paper, Table S3 N/A hPiezo1-pIRES2-EGFP Previously reported in 57 N/A hPiezo1-pIRES2-tdTomato This paper, Table S4 N/A Rat TRPV1 Previously reported in 58 N/A Software and algorithms FIJI (ImageJ) Schindelin et. al. (2012) doi: 10.1038/nmeth.2019 RRID:SCR_002285 Imaris (version 9.9.1) Oxford Instruments RRID:SCR_007370 Prism (GraphPad 9.2.0) GraphPad Software RRID:SCR_002798 Microsoft Excel Microsoft RRID:SCR_016137 FlowJo 10.7.2 BD RRID:SCR_008520 Igor Pro 8.02 Wavemetrics RRID:SCR_000325 pClamp software (version 10.7) Molecular Devices RRID:SCR_011323 Patchmaster HEKA Elektronik RRID:SCR_000034 Other EPC10 amplifier HEKA Elektronik N/A HSPC-1 ALA Scientific Instruments N/A MultiClamp700A amplifier Molecular Devices N/A DigiData1550 Molecular Devices N/A Open in a separate window Key resources table FM 1–43 is a fluorescent dye that labels mouse sensory neurons in vivo. ..
In Vivo:Article Title: Labeling PIEZO2 activity in the peripheral nervous system
Article Snippet: .. Sakura Cat.# 4583 Gold anti-fade mounting medium Thermo Fisher Cat.# S36939 Fluoromount G mounting medium with DAPI Thermo Fisher Cat.# 00-4959-52 Superfrost Plus microscope slides Fisher Cat.# 12-550-15 Lipofectamine 2000 Thermo Fisher Cat.# 11668019 PEI MAX Polysciences Cat.# 24765 Dmem, high glucose, pyruvate Thermo Fisher Cat.# 11995073 Penicillin-Streptomycin (10,000 U/mL) Thermo Fisher Cat.# 15140122 Matrigel Corning Cat.# 354234 MEM (Minimum Essential Medium) Thermo Fisher Cat.# 11095080 Gibco Normal Goat Serum Thermo Fisher Cat.# PCN5000 Donkey Serum Sigma Cat.# D9663 Experimental models: Cell lines Neuro2A-P1ko Gary Lewin 56 N/A Piezo1 knock-out HEK293T (HEK P1KO) ATCC Cat.# CRL-3519 Experimental models: Organisms/strains C57BL/6J Jackson Laboratories RRID:IMSR_JAX:000664 CGRP cre+ ;GFP Mark J. Zylka 35 N/A HoxB8 Cre+ ;Piezo2 f/f Previously reported in 4 N/A Phox2b Cre ; Piezo2 f/f Previously reported in 6 N/A Recombinant DNA hPIEZO2 - codon-optimized-T2A-BFP-WPRE (“PIEZO2”) This paper, Table S1 N/A hPIEZO2 -codon-optimized_mCherry-WPRE This paper, Table S2 N/A hPIEZO2_R2686H -codon-optimized-T2A-BFP-WPRE ( “PIEZO2 R2686H ) This paper, Table S3 N/A hPiezo1-pIRES2-EGFP Previously reported in 57 N/A hPiezo1-pIRES2-tdTomato This paper, Table S4 N/A Rat TRPV1 Previously reported in 58 N/A Software and algorithms FIJI (ImageJ) Schindelin et. al. (2012) doi: 10.1038/nmeth.2019 RRID:SCR_002285 Imaris (version 9.9.1) Oxford Instruments RRID:SCR_007370 Prism (GraphPad 9.2.0) GraphPad Software RRID:SCR_002798 Microsoft Excel Microsoft RRID:SCR_016137 FlowJo 10.7.2 BD RRID:SCR_008520 Igor Pro 8.02 Wavemetrics RRID:SCR_000325 pClamp software (version 10.7) Molecular Devices RRID:SCR_011323 Patchmaster HEKA Elektronik RRID:SCR_000034 Other EPC10 amplifier HEKA Elektronik N/A HSPC-1 ALA Scientific Instruments N/A MultiClamp700A amplifier Molecular Devices N/A DigiData1550 Molecular Devices N/A Open in a separate window Key resources table FM 1–43 is a fluorescent dye that labels mouse sensory neurons in vivo. ..
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